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A transgenic Tbx6;CreER T2 line for inducible gene manipulation in the presomitic mesoderm
Author(s) -
Peter Lopez T.,
Fan ChenMing
Publication year - 2012
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20827
Subject(s) - somitogenesis , paraxial mesoderm , somite , biology , enhancer , mesoderm , transgene , microbiology and biotechnology , intermediate mesoderm , gene , genetics , embryonic stem cell , gene expression
The rhythmic segmentation process of the presomitic mesoderm (PSM) orchestrates the formation of somites, the fundamental units for the vertebrate axial body plan. To aid the investigation of molecular components governing the conversion from PSM into somites, we generated a transgenic mouse line that expresses a tamoxifen (tmx) inducible CreER T2 under the control of a 2.5 kb enhancer element of Tbx6, a gene essential for PSM formation and somite patterning. Combined with Cre reporters, this Tbx6;CreER T2 line displays robust tmx‐inducible Cre activity in the PSM at various embryonic stages. This tool should be useful for studying gene function during somitogenesis by either conditional inactivation or mis‐expression, and potentially coupled with cell marking. genesis 50:490–495, 2011. © 2011 Wiley Periodicals, Inc.