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A transgenic mouse line expressing cre recombinase in pancreatic β‐cells
Author(s) -
Dahlhoff Maik,
Grzech Marjeta,
Habermann Felix A.,
Wolf Eckhard,
Schneider Marlon R.
Publication year - 2012
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20817
Subject(s) - cre recombinase , biology , transgene , genetically modified mouse , recombinase , microbiology and biotechnology , microinjection , cre lox recombination , pancreas , gene , genetics , recombination , biochemistry
Transgenic mouse lines expressing Cre recombinase in a cell‐specific and tissue‐specific manner are essential tools for studying gene function and for developing suitable models for human diseases. Here, we used an expression cassette containing the full 5′ untranslated region of the porcine insulin gene to generate a mouse line expressing Cre recombinase specifically in pancreatic β‐cells by pronuclear DNA microinjection. We obtained a founder animal that transmitted the construct to its descendants in a Mendelian fashion and whose descendants showed a clear activation of β‐galactosidase expression in pancreatic β‐cells after crossing into the ROSA26 lacZ reporter mouse line. Cre expression in other organs was negative except for the kidney, intestine, and the cerebral pons where β‐galactosidase activity was detected in a small percentage of the cells. This new mouse line is a valuable tool for recombination of floxed alleles in pancreatic β‐cells in vivo. genesis 50:437–442, 2012. © 2011 Wiley Periodicals, Inc.