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Gene induction in mature oligodendrocytes with a PLP‐tTA mouse line
Author(s) -
Inamura Naoko,
Sugio Shouta,
Macklin Wendy B.,
Tomita Koichi,
Tanaka Kenji F.,
Ikenaka Kazuhiro
Publication year - 2012
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20808
Subject(s) - oligodendrocyte , proteolipid protein 1 , transgene , biology , microbiology and biotechnology , activator (genetics) , myelin proteolipid protein , myelin , genetically modified mouse , yellow fluorescent protein , green fluorescent protein , gene expression , myelin basic protein , gene , genetics , neuroscience , central nervous system
Abstract Mature oligodendrocytes are critical for myelin maintenance. To understand the molecular basis for this, genetic manipulation of mature oligodendrocytes is needed. Here we generated a mature oligodendrocyte tTA (tetracycline‐controlled transcriptional activator) mouse line which, in combination with a tTA‐dependent promoter line driving the expression of the desired transgene, can be used for gain‐of‐function studies. We used an oligodendrocyte promoter, the mouse proteolipid protein (PLP) promoter, to express mammalianized tTA, and generated a PLP‐mtTA mouse line. In adults, mtTA mRNA was predominantly detected in brain white matter where it co‐localized with PLP mRNA. mtTA‐mediated gene induction was confirmed by crossing to mice with a tTA‐dependent promoter driving expression of yellow fluorescent protein (tetO‐YFP mice). YFP induction in PLP‐mtTA::tetO‐YFP mice was consistent with PLP expression in adult mature oligodendrocytes and premyelinating‐stage myelinating oligodendrocytes. This PLP‐mtTA mouse line is the first to enable gain‐of‐function studies in mature oligodendrocytes with the tet system. genesis 50:424–428, 2012. © 2011 Wiley Periodicals, Inc.