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New Bapx1 Cre‐EGFP mouse lines for lineage tracing and conditional knockout studies
Author(s) -
Sivakamasundari V.,
Yun Chan Hsiao,
Yun Chan Hsiao,
Peng Yap Sook,
Xing Xing,
Kraus Petra,
Lufkin Thomas
Publication year - 2012
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20802
Subject(s) - conditional gene knockout , lineage (genetic) , biology , green fluorescent protein , tracing , knockout mouse , microbiology and biotechnology , genetics , evolutionary biology , gene , phenotype , computer science , operating system
To gain insight into the roles of various genes in development and to circumvent embryonic lethality that hinders genetic studies, lineage tracing and conditional knockout techniques have been widely performed on mice using the increasing numbers of gene‐targeted Cre mouse lines. Employing the internal ribosome entry site (IRES) and the 2A peptide multicistronic expression strategies, we report two new Bapx1 mouse lines with functional Bapx1 whereby Cre and enhanced green fluorescence protein ( EGFP ) are expressed discretely under the control of the Bapx1 promoter. These mouse lines, when mated with the Rosa26R‐lacZ reporter line, can be used to trace the lineage of Bapx1 ‐expressing cells whereas stage‐specific, spatial expression of Bapx1 can be visualized by the EGFP fluorescence. In addition, both of our Bapx1 Cre‐EGFP mouse lines can be used to enrich for Bapx1 ‐specific cells and also serve as effective conditional knockout tools to investigate gene functions in the skeleton and/or visceral organs. genesis, 50:375–383, 2012. © 2012 Wiley Periodicals, Inc.