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Generation of mice with a conditional allele for the transforming growth factor beta3 gene
Author(s) -
Doetschman Thomas,
Georgieva Teodora,
Li Hongqi,
Reed Thomas D.,
Grisham Christina,
Friel Jacqueline,
Estabrook Mark A.,
Gard Connie,
Sanford L.P.,
Azhar Mohamad
Publication year - 2012
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20789
Subject(s) - conditional gene knockout , biology , allele , exon , transforming growth factor , genetics , embryonic stem cell , knockout mouse , gene , cre recombinase , mutation , gene knockout , microbiology and biotechnology , transgene , genetically modified mouse , phenotype
Abstract The transforming growth factor beta (TGFβ) pathway is involved in embryonic development and several inherited and acquired human diseases. The gene for TGFβ3 ( Tgfb3 ) encodes one of the three ligands for TGFβ receptors. It is widely expressed in the embryo and its mutation or misexpression is found in human diseases. Tgfb3 −/− mice die at birth from cleft palate, precluding functional studies in adults. Here, we generated mice in which exon 6 of Tgfb3 was flanked with LoxP sites ( Tgfb3 flox/flox ). The adult mice were normal and fertile. EIIa‐Cre ‐mediated deletion of exon 6 in Tgfb3 flox/flox mice efficiently generated Tgfb3 conditional knockout ( Tgfb3 cko/cko ) mice which died at birth from the same cleft palate defect as Tgfb3 −/− mice, indicating that the conditional and knockout alleles are functionally equivalent. This Tgfb3 cko allele will now enable studies of TGFβ3 function in different cell or tissue types in embryonic development and during adulthood. genesis 50:59–66, 2012. © 2011 Wiley Periodicals, Inc.