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Generation of transgenic mice overexpressing EfnB2 in endothelial cells
Author(s) -
Luxey Maëva,
Laussu Julien,
Jungas Thomas,
Davy Alice
Publication year - 2011
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20785
Subject(s) - angiogenesis , genetically modified mouse , transgene , biology , enhancer , embryonic stem cell , embryo , receptor , microbiology and biotechnology , cancer research , genetics , gene , gene expression
Genetic studies have shown that ephrin‐B2 and its cognate EphB4 receptor are necessary for normal embryonic angiogenesis. Moreover, there is overwhelming evidence that ephrin‐B2 is involved in tumor vascularization, yet its role in adult angiogenesis has been difficult to track genetically. Here, we report the generation of transgenic mice that over‐express EfnB2 specifically in endothelial cells (ECs). We show that exogenous expression of EfnB2 under the control of the Tie2 promoter/enhancer regions in ECs does not affect viability or growth of the transgenic animals. We further show that targeted expression of EfnB2 in ECs is not sufficient to rescue severe cardiovascular defects at mid‐gestation stages but rescues early embryonic lethality associated with loss‐of‐function mutation in EfnB2 . This mouse model will be useful to study the role of ephrin‐B2 in physiological and pathological angiogenesis.genesis 49:811–820, 2011. © 2011 Wiley‐Liss, Inc.