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Offspring from intracytoplasmic sperm injection of aged mouse oocytes treated with caffeine or MG132
Author(s) -
Ono Tetsuo,
Mizutani Eiji,
Li Chong,
Yamagata Kazuo,
Wakayama Teruhiko
Publication year - 2011
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20756
Subject(s) - intracytoplasmic sperm injection , offspring , andrology , human fertilization , caffeine , oocyte , biology , embryo , in vitro fertilisation , sperm , mg132 , pregnancy , endocrinology , anatomy , medicine , genetics , proteasome inhibitor , cell culture
Postovulatory mammalian oocytes age significantly in culture. B6D2F1 or ICR strain mouse oocytes were collected 16 h after hCG injection and then cultured for up to 40 h post hCG at 37°C under 5% CO 2 in air. After intracytoplasmic sperm injection (ICSI), B6D2F1 and ICR oocytes lost full‐term developmental potential by 30 h and 26 h after hCG administration, respectively. However, using supplementation with 10 mM caffeine or 1‐5 μM of MG132, we could obtain live offspring from oocytes at 34 h (BDF1, 5%–21%) or 28 h (ICR, 5%–18%), whereas none were obtained from untreated aged oocytes. Caffeine maintained normal meiotic spindle morphology, whereas MG132 maintained maturation‐promoting factor activity. These treatments did not affect the potential of fresh oocytes for fertilization and subsequent development. Thus, it should be safe to use these chemicals in routine in vitro fertilization and offspring could be generated by ICSI of aged fertilization failed oocytes. genesis 49:460–471, 2011. © 2011 Wiley‐Liss, Inc.