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Stella‐Cre mice are highly efficient Cre deleters
Author(s) -
Liu Hui,
Wang Wei,
Chew SuKit,
Lee SongChoon,
Li Juan,
Vassiliou George S.,
Green Tony,
Futreal P. Andrew,
Bradley Allan,
Zhang Shujun,
Liu Pentao
Publication year - 2011
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20741
Subject(s) - germline , biology , stella (programming language) , cre lox recombination , genetics , gene knockin , locus (genetics) , microbiology and biotechnology , transgene , genetically modified mouse , gene , physics , thermodynamics
Cre‐ lox P recombination is widely used for genetic manipulation of the mouse genome. Here, we report generation and characterization of a new Cre line, Stella‐Cre, where Cre expression cassette was targeted to the 3′ UTR of the Stella locus. Stella is specifically expressed in preimplantation embryos and in the germline. Cre‐ lox P recombination efficiency in Stella‐Cre mice was investigated at several genomic loci including Rosa26 , Jak2 , and Npm1 . At all the loci examined, we observed 100% Cre‐ lox P recombination efficiency in the embryos and in the germline. Thus, Stella‐Cre mice serve as a very efficient deleter line. genesis 49:689–695, 2011. © 2011 Wiley‐Liss, Inc.