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Generation and characterization of iUBC‐KikGR photoconvertible transgenic mice for live time‐lapse imaging during development
Author(s) -
Griswold Shan L.,
Sajja Krishna C.,
Jang ChuanWei,
Behringer Richard R.
Publication year - 2011
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20718
Subject(s) - genetically modified mouse , transgene , biology , microbiology and biotechnology , green fluorescent protein , embryo , fate mapping , gene , stem cell , genetics , progenitor cell
A transgenic mouse line named iUBC‐KikGR was generated, which expresses the photoconvertible fluorescent protein Kikume Green‐Red (KikGR) under the control of the human Ubiquitin C promoter. KikGR is natively a green fluorophore, which can be converted into a red fluorophore upon exposure to UV light. KikGR is expressed broadly throughout transgenic embryos from the two‐cell stage onward and in the adult. Specificity of photoconversion can range from the entire embryo to a region of an organ, to a few individual cells, depending on the needs of the experimenter. Cell movements, tissue reorganization, and migration can then be observed in real time by culturing the tissue of interest as an explant on the microscope stage. The iUBC‐KikGR transgenic line represents a singular genetic reagent, which can be used for fate mapping, lineage tracing, and live visualization of cell behaviors and tissue movements in multiple organs at multiple time points. genesis 49:591–598, 2011. © 2011 Wiley‐Liss, Inc.