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Use of fully modified 2′‐O‐methyl antisense oligos for loss‐of‐function studies in vertebrate embryos
Author(s) -
Schneider Patricia N.,
Olthoff John T.,
Matthews Abby J.,
Houston Douglas W.
Publication year - 2011
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20689
Subject(s) - morpholino , xenopus , zebrafish , oligonucleotide , biology , chordin , translation (biology) , microbiology and biotechnology , gene , embryo , function (biology) , genetics , messenger rna , embryogenesis , gastrulation
Antisense oligonucleotides are commonly employed to study the roles of genes in development. Although morpholino phosphorodiamidate oligonucleotides (morpholinos) are widely used to block translation or splicing of target gene products' the usefulness of other modifications in mediating RNase‐H independent inhibition of gene activity in embryos has not been investigated. In this study, we investigated the extent that fully modified 2′‐O‐methyl oligonucleotides (2′‐OMe oligos) that can function as translation inhibiting reagents in vivo, using Xenopus and zebrafish embryos. We find that oligos against Xenopus β‐ catenin , wnt11 , and bmp4 and against zebrafish chordin ( chd ), which can efficiently and specifically generate embryonic loss‐of‐function phenotypes comparable with morpholino injection and other methods. These results show that fully modified 2′‐OMe oligos can function as RNase‐H independent antisense reagents in vertebrate embryos and can thus serve as an alternative modification to morpholinos in some cases. genesis 49:117–123, 2011. © 2010 Wiley‐Liss, Inc.