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Characterization of a mouse Scube3 reporter line
Author(s) -
Xavier Guilherme M.,
Economou Andrew,
Senna Guimarães André Luis,
Sharpe Paul T.,
Cobourne Martyn T.
Publication year - 2010
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20678
Subject(s) - biology , exon , neural tube , gene , signal peptide , coding region , microbiology and biotechnology , reporter gene , genetics , peptide sequence , embryo , gene expression
Abstract The SCUBE gene family encode secreted, extracellular proteins that share a distinct domain organization of at least five recognizable motifs, including an amino‐terminal signal peptide sequence, multiple EGF‐like domains, a large spacer region containing multiple N ‐linked glycosylation sites, three repeated stretches of six‐cysteine residues and a carboxy‐terminal CUB domain. We describe a Scube3 tm1Dge/H targeted allele, which replaces the entire coding region for Exons 2 and 3 with a neomycin‐lacZ selectable marker cassette predicted to delete the first two EGF‐like domains of the transcribed protein. Scube3 +/ tm1Dge/H embryos demonstrate strong β‐galactosidase activity in the early facial epithelium, including the branchial arches and facial processes, the otic vesicle, limb buds, and neural tube. In addition, strong reporter activity was identified in the epithelial compartments of developing teeth and hair follicles. However, analysis of the Scube3 tm1Dge/H allele revealed that it encodes a truncated protein, which contains part of the spacer region and CUB domain. It is likely that this protein retains functionality because our analysis reveals that Scube3 tm1Dge/H; tm1Dge/H mice are phenotypically normal. Whilst acting as a useful reporter, these mice do not provide any insight into the potential role of Scube3 during embryonic development. genesis 48:684–692, 2010. © 2010 Wiley‐Liss, Inc.