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The contribution of the Tie2 + lineage to primitive and definitive hematopoietic cells
Author(s) -
Tang Yuefeng,
Harrington Anne,
Yang Xuehui,
Friesel Robert E.,
Liaw Lucy
Publication year - 2010
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20654
Subject(s) - biology , haematopoiesis , bone marrow , yolk sac , lineage (genetic) , hemangioblast , progenitor cell , population , microbiology and biotechnology , stem cell , immunology , genetics , gene , embryo , medicine , environmental health
The regulatory elements of the Tie2 / Tek promoter are commonly used in mouse models to direct transgene expression to endothelial cells. Tunica intima endothelial kinase 2 ( Tie2 ) is also expressed in hematopoietic cells, although this has not been fully characterized. We determine the lineages of adult hematopoietic cells derived from Tie2 ‐expressing populations using Tie2‐Cre;Rosa26R‐EYFP mice. In Tie2‐Cre;Rosa26R‐EYFP mice, analysis of bone marrow cells showed Cre‐mediated recombination in 85% of the population. In adult bone marrow and spleen, we analyzed subclasses of early hematopoietic progenitors, T cells, monocytes, granulocytes, and B cells. We found that ∼ 84% of each lineage was EYFP + , and nearly all cells that come from Tie2‐expressing lineages are CD45 + , confirming widespread contribution to definitive hematopoietic cells. In addition, more than 82% of blood cells within the embryonic yolk sac were of Tie2 + origin. Our findings of high levels of Tie2‐Cre recombination in the hematopoietic lineage have implications for the use of the Tie2‐Cre mouse as a lineage‐restricted driver strain. genesis 48:563–567, 2010. © 2010 Wiley‐Liss, Inc.