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A unique expression pattern of Tbx10 in the hindbrain as revealed by Tbx10LacZ allele
Author(s) -
Xue Xiao Dong,
Kimura Wataru,
Wang Bo,
Hikosaka Keisuke,
Itakura Tatsuo,
Uezato Tadayoshi,
Matsuda Masashi,
Koseki Haruhiko,
Miura Naoyuki
Publication year - 2010
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20643
Subject(s) - rhombomere , biology , hindbrain , lac operon , embryonic stem cell , microbiology and biotechnology , gene , pons , gene expression , genetics , anatomy , hox gene
To study the expression/function of Tbx10 , a T‐box gene, Tbx10LacZ/+ mice were established by replacing the T‐box coding region with a LacZ gene. X‐gal staining showed that LacZ+ cells were localized to two‐cell populations in rhombomere 4 and rhombomere 6. No significant differences in the locations of LacZ+ cells were found between Tbx10LacZ/+ and Tbx10LacZ/LacZ mice, and the Tbx10LacZ/LacZ mice were viable and fertile. We found that the LacZ+ cells are present in both embryonic and adult mice. Histological studies suggest that the rhombomere 4‐derived LacZ+ cells are a subpopulation of the ventral interneurons in the pons.