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Dynamic expression of a LEF‐EGFP Wnt reporter in mouse development and cancer
Author(s) -
Currier Nicolas,
Chea Kathleen,
Hlavacova Mirka,
Sussman Daniel J.,
Seldin David C.,
Dominguez Isabel
Publication year - 2010
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20604
Subject(s) - wnt signaling pathway , green fluorescent protein , biology , transgene , genetically modified mouse , microbiology and biotechnology , primitive streak , embryogenesis , signal transduction , embryo , gene , genetics , gastrulation
We have characterized a transgenic mouse line in which enhanced green fluorescent protein (EGFP) is expressed under the control of multimerized LEF‐1 responsive elements. In embryos, EGFP was detected in known sites of Wnt activation, including the primitive streak, mesoderm, neural tube, somites, heart, limb buds, mammary placodes, and whisker follicles. In vitro cultured transgenic embryonic fibroblasts upregulated EGFP expression in response to activation of Wnt signaling by GSK3β inhibition. Mammary tumor cell lines derived from female LEF‐EGFP transgenic mice treated with the carcinogen 7, 12‐dimethylbenz[a]anthracene (DMBA) also express EGFP. Thus, this transgenic line is useful for ex vivo and in vitro studies of Wnt signaling in development and cancer. genesis 48:183–194, 2010. © 2010 Wiley‐Liss, Inc.