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A mouse model to dissect progesterone signaling in the female reproductive tract and mammary gland
Author(s) -
FernandezValdivia Rodrigo,
Jeong Jaewook,
Mukherjee Atish,
Soyal Selma M.,
Li Jie,
Ying Yan,
DeMayo Francesco J.,
Lydon John P.
Publication year - 2010
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20586
Subject(s) - mammary gland , reproductive tract , female reproductive tract , biology , progesterone receptor , microbiology and biotechnology , endocrinology , medicine , uterus , genetics , estrogen receptor , cancer , breast cancer
Considering the regulatory complexities of progesterone receptor (PR) action throughout the female reproductive axis and mammary gland, we generated a mouse model that enables conditional ablation of PR function in a spatiotemporal specific manner. Exon 2 of the murine PR gene was floxed to generate a conditional PR allele (PR flox ) in mice. Crossing the PR flox/flox mouse with the ZP3‐cre transgenic demonstrated that the PR flox allele recombines to a PR null allele (PR d ). Mice homozygous for the recombined null PR allele (PR d/d ) exhibit uterine, ovarian, and mammary gland defects that phenocopy those of our previously described PR knockout (PRKO) model. Therefore, this conditional mouse model for PR ablation represents an invaluable resource with which to further define in a developmental and/or reproductive stage‐specific manner the individual and integrative roles of distinct PR populations resident in multiple progesterone‐responsive target sites. genesis 48:106–113, 2010. © 2009 Wiley‐Liss, Inc.