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TDP‐43, a neuro‐pathosignature factor, is essential for early mouse embryogenesis
Author(s) -
Wu LienSzu,
Cheng WeiCheng,
Hou ShinChen,
Yan YuTing,
Jiang SiTse,
Shen C.K. James
Publication year - 2010
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20584
Subject(s) - biology , microbiology and biotechnology , amyotrophic lateral sclerosis , transcription factor , embryo , translation (biology) , tardbp , embryogenesis , embryonic stem cell , rna splicing , gene , genetics , mutant , messenger rna , sod1 , disease , pathology , medicine , rna
TDP‐43 is a highly conserved and ubiquitously expressed nuclear protein. It has been implicated in the regulation of transcription, alternative splicing, translation, and neuronal plasticity. TDP‐43 has also been shown to be a disease signature protein associated with several neurodegenerative diseases including amyotrophic lateral sclerosis. However, the correlation of the physiological functions of TDP‐43 with these diseases remains unknown. We have used the gene targeting approach to disrupt the expression of TDP‐43 in mouse. Loss of the TDP‐43 expression results in peri‐implantation lethality of mice between embryonic days (E) 3.5 and 6.5. Blastocysts of the homozygous Tardbp null mutants are morphologically normal, but exhibit defective outgrowth of the inner cell mass in vitro. Our data demonstrate the essential function of TDP‐43 in peri‐implantation stage during the embryo development, likely because of its involvement in multiple biological processes in a variety of cell types. genesis 48:56–62, 2010. © 2009 Wiley‐Liss, Inc.