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Dual fluorescent protein reporters for studying cell behaviors in vivo
Author(s) -
Stewart M. David,
Jang ChuanWei,
Hong Nicholas W.,
Austin Anthony P.,
Behringer Richard R.
Publication year - 2009
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20582
Subject(s) - green fluorescent protein , fluorescence , yellow fluorescent protein , hela , transfection , microbiology and biotechnology , membrane , fluorescent protein , biology , in vivo , nuclear localization sequence , cell , cytoplasm , chemistry , gene , biochemistry , genetics , quantum mechanics , physics
Abstract HeLa cells transfected with plasmids expressing dual fluorescent protein reporters with cherry, green, and cyan fluorscent proteins. Histone 2B fusions localize fluorescent proteins to the nucleus, while fluorescent proteins fused to glycosylphosphatidylinositol anchor signal sequence localizes to plasma membranes. These dual fluorescent reporters label nuclear and plasma membranes of live cells to follow their behaviors in developing embryos. See the paper by Stewart et al. in this issue.