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An estrogen receptor alpha activity indicator model in mice
Author(s) -
Han Sang Jun,
O'Malley Bert W.,
DeMayo Francesco J.
Publication year - 2009
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20572
Subject(s) - reporter gene , estrogen receptor , biology , estrogen receptor beta , estrogen receptor alpha , transgene , microbiology and biotechnology , hormone response element , transcription factor , estrogen , gene , bacterial artificial chromosome , clone (java method) , gene expression , genetics , cancer , genome , breast cancer
Estrogen receptor alpha (ERα), plays essential roles in the female reproduction. To investigate the dynamic changes in ERα activity in vivo, we have developed an ER Alpha Activity Indicator (ERAAI) mouse. This ERAAI mouse harbors both a modified ERα Bacterial Artificial Chromosome (BAC) clone and a reporter gene which is regulated specifically by the modified receptor. The ERα modification (Gal4‐ERα) consists replacing the DNA binding domain (DBD) of ERα with the DBD of yeast Gal4 transcription factor. This reporter transgene consisting of a humanized renilla Green Fluorescent Protein (hrGFP) sequence controlled by the U pstream A ctivating S equences for the G al4 gene (UAS G ) was inserted into the modified ERα BAC clone. Expression of Gal4‐ERα and hrGFP reliably recapitulates endogenous ERα expression and activity in the estrogen target tissues in response to estrogen stimulation. Therefore, the ERAAI mouse represents a novel animal model to investigate dynamic ERα activity in vivo. genesis 47:815–824, 2009. © 2009 Wiley‐Liss, Inc.