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Developmental regulation of the composite CAG promoter activity in the murine T lymphocyte cell lineage
Author(s) -
Baup Delphine,
Moser Muriel,
Schurmans Stéphane,
Leo Oberdan
Publication year - 2009
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20569
Subject(s) - biology , transgene , cd8 , genetically modified mouse , lineage (genetic) , t cell , gene , microbiology and biotechnology , promoter , green fluorescent protein , lymphocyte , downregulation and upregulation , t lymphocyte , in vivo , gene expression , immunology , genetics , immune system
Promoter selection is of utmost importance for the study of in vivo gene function using transgenic models. In the present study, we have analyzed the expression of the GFP marker under the control of the composite CAG promoter in the lymphoid compartment of several transgenic mouse strains. Despite the ability of the CAG promoter to drive gene expression in almost all tissues examined to date, its activity appears to be developmentally regulated within the T lymphocyte cell lineage. In particular, CD4 and CD8‐expressing, thymic immature T cells displayed lower levels of the GFP marker when compared with both bone marrow precursors and mature circulating T cells, suggesting a transient downregulation of CAG activity during T cell development. Alternative promoters may therefore be preferred for the study of T cell development in vivo using a transgenic approach. genesis 47:799–804, 2009. © 2009 Wiley‐Liss, Inc.