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Generation and analysis of a mouse line harboring GFP in the Eomes/Tbr2 locus
Author(s) -
Arnold Sebastian J.,
Sugnaseelan Jacintha,
Groszer Matthias,
Srinivas Shankar,
Robertson Elizabeth J.
Publication year - 2009
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20562
Subject(s) - endoderm , biology , primitive streak , mesoderm , progenitor cell , allele , transcription factor , progenitor , embryonic stem cell , microbiology and biotechnology , genetics , gene , stem cell
During mouse embryonic development, the T‐box transcription factor Eomes/Tbr2 is expressed in highly dynamic patterns in various progenitor cell types. Those include the undifferentiated cells of the trophectoderm, ingressing nascent mesoderm at the primitive streak, and intermediate progenitor cells of the developing cerebral cortex. We generated an Eomes GFP ‐ targeted allele to follow the highly dynamic patterns of Eomes expression and to allow for the identification of novel expression domains. We show that our novel allele recapitulates endogenous gene expression at known sites of expression and confirm our results by anti‐Eomes immunofluorescent staining. Using this novel allele we were able to identify previously undocumented domains of Eomes expression within the visceral endoderm and at various locations in the developing and adult mouse brain. genesis 47:775–781, 2009. © 2009 Wiley‐Liss, Inc.