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New hemocyte‐specific enhancer‐reporter transgenes for the analysis of hematopoiesis in Drosophila
Author(s) -
Tokusumi Tsuyoshi,
Shoue Douglas A.,
Tokusumi Yumiko,
Stoller Jessica R.,
Schulz Robert A.
Publication year - 2009
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20561
Subject(s) - enhancer , biology , transgene , reporter gene , drosophila (subgenus) , gene , haematopoiesis , microbiology and biotechnology , green fluorescent protein , drosophila melanogaster , genetics , stem cell , gene expression
Based on environmental challenges or altered genetic composition, Drosophila larvae can produce up to three types of blood cells that express genetic programs essential for their distinct functions. Using transcriptional enhancers for genes expressed exclusively in plasmatocytes, crystal cells, or lamellocytes, several new hemocyte‐specific enhancer‐reporter transgenes were generated to facilitate the analysis of Drosophila hematopoiesis. This approach took advantage of fluorescent variants of insulated P‐element reporter vectors for multilabeling cell analyses; two additional color variants were generated in these studies. These vectors were successfully used to produce transgenic fly lines that label specific hemocyte lineages with separate colors. Combining three transgene reporters allowed for the unambiguous identification of plasmatocytes, crystal cells, and lamellocytes within a complex hemocyte population. While this work focused on the hematopoietic process, these new vectors can be used to mark multiple cell types or trace complex cell lineages during any chosen aspect of Drosophila development. genesis 47:771–774, 2009. © 2009 Wiley‐Liss, Inc.