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Generation of a conditional CREB Ser133Ala knockin mouse
Author(s) -
Wingate Andrew D.,
Martin Kirsty J.,
Hunter Chris,
Carr Julia M.,
Clacher Carol,
Arthur J. Simon C.
Publication year - 2009
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20548
Subject(s) - creb , biology , cre recombinase , minigene , transcription factor , transcription (linguistics) , conditional gene knockout , genetics , microbiology and biotechnology , genetically modified mouse , transgene , phenotype , gene , messenger rna , alternative splicing , linguistics , philosophy
Phosphorylation of Ser133 in the transcription factor CREB is an important mechanism for regulating its transcriptional activity, however recent work has suggested significant roles for other regulatory inputs into CREB. To allow study of this in vivo, we have generated a Ser133 to alanine knockin mutation in the mouse CREB locus. As CREB knockout is perinatal lethal, a minigene strategy was used to allow conditional knockin of the Ser133Ala mutation in adult mice using Cre recombinase. While some expression of the mutated protein was observed prior to Cre expression, following Cre expression in either T cells or neurons only the mutated CREB protein was detected. genesis 47:688–696, 2009. © 2009 Wiley‐Liss, Inc.