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A transgenic mouse that reveals cell shape and arrangement during ureteric bud branching
Author(s) -
Chi Xuan,
Hadjantonakis AnnaKaterina,
Wu Zaiqi,
Hyink Deborah,
Costantini Frank
Publication year - 2009
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20523
Subject(s) - ureteric bud , deconvolution , confocal , genetically modified mouse , biology , rendering (computer graphics) , embryonic stem cell , lens (geology) , transgene , anatomy , kidney development , optics , computer science , computer graphics (images) , physics , paleontology , biochemistry , gene
3‐D images showing the outlines of individual cells in the ureteric bud tips of a Hoxb7/myr‐Venus transgenic mouse kidney at embryonic day 15.5. Image stacks were acquired with a Bio‐Rad laser scanning confocal microscope equipped with an Olympus U PlanApo/IR water lens 60x /NA1.2. The images were processed using blind deconvolution (AutoDeblur, Media Cybernetics, Bethesda, MD), followed by volume rendering (Volocity software). See the article by Chi et al. in this issue.