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Generation of mice with a conditional allele for transforming growth factor beta 1 gene
Author(s) -
Azhar Mohamad,
Yin Moying,
Bommireddy Ramireddy,
Duffy John J.,
Yang Junqi,
Pawlowski Sharon A.,
Boivin Gregory P,
Engle Sandra J.,
Sanford L.P.,
Grisham Christina,
Singh Ram R.,
Babcock George F.,
Doetschman Thomas
Publication year - 2009
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20516
Subject(s) - conditional gene knockout , gene knockout , transforming growth factor , transforming growth factor beta , biology , knockout mouse , pathogenesis , autoimmunity , allele , genetically modified mouse , immunology , gene targeting , transgene , gene knockin , t cell , cancer research , gene , microbiology and biotechnology , genetics , phenotype , immune system
Transforming growth factor β1 (TGFβ1) is a multifunctional growth factor involved in wound healing, tissue fibrosis, and in the pathogenesis of many syndromic diseases (e.g., Marfan syndrome, Camurati‐Engelmann disease) and muscular, neurological, ophthalmic, cardiovascular and immunological disorders, and cancer. Since the generation of Tgfb1 knockout mice, there has been extraordinary progress in understanding its physiological and pathophysiological function. Here, we report the generation of a conditional knockout allele for Tgfb1 in which its exon 6 is flanked with LoxP sites. As proof of principle, we crossed these mice to LckCre transgenic mice and specifically disrupted Tgfb1 in T cells. The results indicate that T‐cell‐produced TGFβ1 is required for normal in vivo regulation of peripheral T‐cell activation, maintenance of T‐cell homeostasis, and suppression of autoimmunity. genesis 47:423–431, 2009. © 2009 Wiley‐Liss, Inc.