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Cre/lox recombination in the lower urinary tract
Author(s) -
Wang Yinqiu,
Tripathi Piyush,
Guo Qiusha,
Coussens Matthew,
Ma Liang,
Chen Feng
Publication year - 2009
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20515
Subject(s) - transgene , urothelium , biology , urinary system , microbiology and biotechnology , genetically modified mouse , stromal cell , endogeny , gene , genetics , anatomy , cancer research , endocrinology
Tbx18 is a T‐Box transcription factor that has specific expression and indispensible function in the lower urinary tract. Here, we report the generation and characterization of a bacterial artificial chromosome (BAC) transgene expressing Cre under the control of Tbx18 regulatory elements. When crossed to the ROSA26R‐lacZ reporter mice, the Tbx18‐Cre transgene mediates loxP recombination in the mesenchymal derivatives in the lower urinary tract, especially in the smooth muscle cells (SMCs) and the stromal cells. There is no expression of this transgene in the urothelium or in the kidney. This Tbx18‐Cre transgene recapitulates the endogenous Tbx18 expression in the urinary system and can be used for the study of the development, physiology, and diseases in the urinary tract. Its additional expression in the epicardium, limb, vibrissae, and other structures would be useful for studies in the relevant fields. genesis 47:409–413, 2009. © 2009 Wiley‐Liss, Inc.