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Generation of red fluorescent protein transgenic dogs
Author(s) -
Hong So Gun,
Kim Min Kyu,
Jang Goo,
Oh Hyun Ju,
Park Jung Eun,
Kang Jung Taek,
Koo Ok Jae,
Kim Teoan,
Kwon Mo Sun,
Koo Bon Chul,
Ra Jeong Chan,
Kim Dae Yong,
Ko CheMyong,
Lee Byeong Chun
Publication year - 2009
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20504
Subject(s) - green fluorescent protein , transgene , biology , retrovirus , transfection , embryonic stem cell , somatic cell nuclear transfer , somatic cell , microbiology and biotechnology , genetically modified mouse , gene , stem cell , embryo , genetics , embryogenesis , blastocyst
Dogs ( Canis familiaris ) share many common genetic diseases with humans and development of disease models using a transgenic approach has long been awaited. However, due to the technical difficulty in obtaining fertilizable eggs and the unavailability of embryonic stem cells, no transgenic dog has been generated. Canine fetal fibroblasts were stably transfected with a red fluorescent protein ( RFP ) gene‐expressing construct using retrovirus gene delivery method. Somatic cell nuclear transfer was then employed to replace the nucleus of an oocyte with the nucleus of the RFP ‐fibroblasts. Using this approach, we produced the first generation of transgenic dogs with four female and two male expressing RFP . genesis 47:314–322, 2009. © 2009 Wiley‐Liss, Inc.