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Generation of transgenic mice expressing Cre recombinase under the control of the Dll1 mesoderm enhancer element
Author(s) -
Wehn Amy K.,
Gallo Phillip H.,
Chapman Deborah L.
Publication year - 2009
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20503
Subject(s) - paraxial mesoderm , mesoderm , nodal , intermediate mesoderm , biology , cre recombinase , enhancer , genetically modified mouse , lateral plate mesoderm , fgf and mesoderm formation , endoderm , microbiology and biotechnology , recombinase , transgene , embryonic stem cell , genetics , gene , gene expression , recombination
Abstract To study paraxial mesoderm formation in the mouse, transgenic lines that can be used to either selectively delete or express genes of interest in the paraxial mesoderm are required. We have generated a transgenic mouse line that expresses Cre recombinase in the paraxial mesoderm (PAM) beginning at e7.5. A lacZ Cre recombinase reporter line showed that in addition to PAM and its derivatives, lateral plate and intermediate mesoderm derivatives were also exposed to Cre activity, while the node, notochord, and cardiac mesoderm were not. We further demonstrate that 70–75% of the fibroblasts generated from Dll1‐msd Cre , ROSA26‐rtTA embryos possess Cre recombinase activity. These mice can therefore be used in combination with tet‐responsive transgenic lines to generate mesoderm‐derived embryonic fibroblasts that inducibly express a gene of interest. genesis 47:309–313, 2009. © 2009 Wiley‐Liss, Inc.