Molecular cloning of a dominant roller mutant and establishment of DNA‐mediated transformation in the nematode  Pristionchus pacificus | Zendy

Schlager Benjamin | Zendy; Wang Xiaoyue | Zendy; Braach Georg | Zendy; Sommer Ralf J. | Zendy

Premium

Molecular cloning of a dominant roller mutant and establishment of DNA‐mediated transformation in the nematode Pristionchus pacificus

Author(s) -

Schlager Benjamin,

Wang Xiaoyue,

Braach Georg,

Sommer Ralf J.

Publication year - 2009

Publication title -

genesis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.093

H-Index - 110

eISSN - 1526-968X

pISSN - 1526-954X

DOI - 10.1002/dvg.20499

Subject(s) - mutant , cloning (programming) , biology , transformation (genetics) , transgene , molecular cloning , positional cloning , locus (genetics) , dna , genetics , nematode , microbiology and biotechnology , gene , complementary dna , ecology , computer science , programming language

We report the molecular cloning of a dominant Roller mutant of Pristionchus pacificus , which encodes a cuticle collagen. We use the mutant locus as a marker to develop transgenic technique by generating complex arrays and present flourescent‐protein based transcriptional reporter constructs for P. pacificus . genesis 47:300–304, 2009. © 2009 Wiley‐Liss, Inc.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research