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Molecular cloning of a dominant roller mutant and establishment of DNA‐mediated transformation in the nematode Pristionchus pacificus
Author(s) -
Schlager Benjamin,
Wang Xiaoyue,
Braach Georg,
Sommer Ralf J.
Publication year - 2009
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20499
Subject(s) - mutant , cloning (programming) , biology , transformation (genetics) , transgene , molecular cloning , positional cloning , locus (genetics) , dna , genetics , nematode , microbiology and biotechnology , gene , complementary dna , ecology , computer science , programming language
We report the molecular cloning of a dominant Roller mutant of Pristionchus pacificus , which encodes a cuticle collagen. We use the mutant locus as a marker to develop transgenic technique by generating complex arrays and present flourescent‐protein based transcriptional reporter constructs for P. pacificus . genesis 47:300–304, 2009. © 2009 Wiley‐Liss, Inc.