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A multifunctional reporter mouse line for Cre‐ and FLP‐dependent lineage analysis
Author(s) -
Yamamoto Masakazu,
Shook Nicole A.,
Kanisicak Onur,
Yamamoto Shoko,
Wosczyna Michael N.,
Camp James R.,
Goldhamer David J.
Publication year - 2009
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20474
Subject(s) - germline , transgene , gene knockin , biology , site specific recombination , reporter gene , cre recombinase , microbiology and biotechnology , green fluorescent protein , chromatin , genetically modified mouse , recombinase , genetics , recombination , gene , gene expression
The Cre/lox and FLP/FRT recombination systems have been used extensively for both conditional knockout and cell lineage analysis in mice. Here we report a new multifunctional Cre/FLP dual reporter allele ( R26 NZG ) that exhibits strong and apparently ubiquitous marker expression in embryos and adults. The reporter construct, which is driven by the CAG promoter, was knocked into the ROSA26 locus providing an open chromatin domain for consistent expression and avoiding site‐of‐integration effects often observed with transgenic reporters. R26 NZG directs Cre‐dependent nuclear‐localized β‐galactosidase (β‐gal) expression, and can be converted into a Cre‐dependent EGFP reporter ( R26 NG ) by germline excision of the FRT‐flanked nlslacZ cassette. Alternatively, germline excision of the floxed PGKNEO cassette in R26 NZG generates an FLP‐dependent EGFP reporter ( R26 ZG ) that expresses β‐gal in FLP‐nonexpressing cells. Finally, by the simultaneous use of both Cre and FLP deleters, R26 NZG allows lineage relationships to be interrogated with greater refinement than is possible with single recombinase reporter systems. genesis 47:107–114, 2009. © 2009 Wiley‐Liss, Inc.