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Novel caspase‐suicide proteins for tamoxifen‐inducible apoptosis
Author(s) -
Chu Yuanyuan,
Senghaas Niklas,
Köster Reinhard W.,
Wurst Wolfgang,
Kühn Ralf
Publication year - 2008
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20426
Subject(s) - zebrafish , apoptosis , microbiology and biotechnology , fusion protein , caspase , estrogen receptor , biology , tamoxifen , caspase 8 , ligand (biochemistry) , programmed cell death , cancer research , chemistry , receptor , gene , genetics , cancer , recombinant dna , breast cancer
Taking advantage of a mutant estrogen receptor ligand binding domain (ER T2 ), we developed novel Caspase fusion proteins for inducible apoptosis. We show that Caspase‐ER T2 fusion proteins become specifically activated by the synthetic ligand 4‐OH‐ tamoxifen and rapidly induce apoptotic cell death in human, murine, and zebrafish cells. This novel tool for targeted cell ablation greatly facilitates the generation of disease models as well as developmental and regeneration studies in model organisms. genesis 46:530–536, 2008. © 2008 Wiley‐Liss, Inc.