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Analysis of CRE‐mediated recombination driven by myosin light chain 1/3 regulatory elements in embryonic and adult skeletal muscle: A tool to study fiber specification
Author(s) -
Mourkioti Foteini,
Slonimsky Esfir,
Huth Marion,
Berno Valeria,
Rosenthal Nadia
Publication year - 2008
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20419
Subject(s) - embryonic stem cell , myosin , microbiology and biotechnology , skeletal muscle , immunoglobulin light chain , biology , skeletal muscle fibers , anatomy , genetics , gene , antibody
An increasing number of genes have been implicated in skeletal muscle fiber diversity. To study the contribution of diverse genetic elements to the regulation of fiber‐type composition, we generated a transgenic mouse in which CRE recombinase expression is driven by muscle‐specific regulatory sequences of the myosin light chain 1/3 locus (MLC). Using ROSA26 conditional reporter mice, we detected expression of the MLC‐Cre transgene starting from embryonic day 12.5 (E12.5). By E15, recombination was detected in all muscle‐derived structures. Immunohistochemical analysis revealed CRE activity was restricted to fast‐twitch (type II) and excluded from slow‐twitch (type I) fibers of skeletal muscle. The MLC‐Cre transgenic mouse can be used in conjunction with conditional alleles to study both developmental patterning and maintenance of fast fiber‐type phenotypes. genesis 46:424–430, 2008. © 2008 Wiley‐Liss, Inc.