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Sfrp1, Sfrp2, and Sfrp5 regulate the Wnt/β‐catenin and the planar cell polarity pathways during early trunk formation in mouse
Author(s) -
Satoh Wataru,
Matsuyama Makoto,
Takemura Hiromasa,
Aizawa Shinichi,
Shimono Akihiko
Publication year - 2008
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20369
Subject(s) - wnt signaling pathway , biology , dkk1 , microbiology and biotechnology , somitogenesis , mutant , convergent extension , somite , cell fate determination , embryo , genetics , signal transduction , embryogenesis , gene , gastrulation , transcription factor
Sfrp is a secreted Wnt antagonist that directly interacts with Wnt ligand. We show here that inactivation of Sfrp1 , Sfrp2 , and Sfrp5 leads to fused somites formation in early‐somite mouse embryos, simultaneously resulting in defective convergent extension (CE), which causes severe shortening of the anteroposterior axis. These observations indicate the redundant roles of Sfrp1 , Sfrp2 , and Sfrp5 in early trunk formation. The roles of the Sfrps were genetically distinguished in terms of the regulation of Wnt pathways. Genetic analysis combining Sfrps mutants and Loop‐tail mice revealed the involvement of Sfrps in CE through the regulation of the planar cell polarity pathway. Furthermore, Dkk1 ‐deficient embryos carrying Sfrp1 homozygous and Sfrp2 heterozygous mutations display irregular somites and indistinct intersomitic boundaries, which indicates that Sfrps‐mediated inhibition of the Wnt/β‐catenin pathway is necessary for somitogenesis. Our results suggest that Sfrps regulation of the canonical and noncanonical pathways is essential for proper trunk formation. genesis 46:92–103, 2008. © 2008 Wiley‐Liss, Inc.