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A Cre transgene active in developing endodermal organs, heart, limb, and extra‐ocular muscle
Author(s) -
Grieshammer Uta,
Agarwal Pooja,
Martin Gail R.
Publication year - 2008
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20366
Subject(s) - biology , mesenchyme , cre recombinase , foregut , transgene , microbiology and biotechnology , endoderm , mesoderm , limb bud , anatomy , quail , genetically modified mouse , embryonic stem cell , endocrinology , embryo , genetics , gene
Cre‐mediated recombination, a method widely used in mice for tissue‐specific inactivation of endogenous genes or activation of transgenes, is critically dependent on the availability of mouse lines in which Cre recombinase functions in the tissue of interest or its progenitors. Here we describe a transgenic mouse line, Osr1‐ cre, in which Cre is active from embryonic day (E)11.5 in a few specific tissues. These include the endoderm of the posterior foregut, midgut, hindgut, and developing urogenital system, the heart left atrium, extra‐ocular muscle progenitors, and mesenchyme in particular regions of the limb. Furthermore, starting at E12.5, Cre functions in limb interdigital mesenchyme. Within the urogenital system, recombination appears to be virtually complete in the epithelium of the bladder and urethra just posterior to it by E14.5. In males, some of these urethral cells form the prostate. The spatiotemporal pattern of Cre activity in Osr1 ‐cre makes it a unique resource among the lines available for Cre‐mediated recombination experiments. genesis 46:69–73, 2008. © 2008 Wiley‐Liss, Inc.