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Foxb1 ‐driven Cre expression in somites and the neuroepithelium of diencephalon, brainstem, and spinal cord
Author(s) -
Zhao Tianyu,
Zhou Xunlei,
Szabó Nora,
Leitges Michael,
AlvarezBolado Gonzalo
Publication year - 2007
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20356
Subject(s) - biology , diencephalon , cre recombinase , brainstem , green fluorescent protein , neuroepithelial cell , spinal cord , fate mapping , embryonic stem cell , anatomy , microbiology and biotechnology , central nervous system , neuroscience , genetically modified mouse , transgene , genetics , gene
We have knocked‐in Cre‐IRES‐EGFP in the Foxb1 locus by homologous recombination in embryonic stem cells. We removed the PGK‐neo cassette (which was flanked by FRT sequences) by crossing with the FLPeR deleter mouse. The Foxb1 Cre line showed Cre recombinase activity as well as EGFP fluorescence reproducing Foxb1 expression accurately. By crossing Foxb1 Cre mice with the ROSA26R and Z/AP mouse reporter lines we have been able to trace the lineage of Foxb1 ‐expressing cells. Early transient expression of Foxb1 in the paraxial mesoderm translates into labeling of the somites. In the central nervous system (CNS), the Foxb1 lineage includes the thalamus and mammillary body (hypothalamus), brainstem, and the ventral spinal cord and floor plate. genesis 45:781–787, 2007. © 2007 Wiley‐Liss, Inc.