Premium
Transgenic mouse lines expressing Cre recombinase specifically in posterior notochord and notochord
Author(s) -
Kumar Amit,
Yamaguchi Taihei,
Sharma Prashant,
Kuehn Michael R.
Publication year - 2007
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20346
Subject(s) - notochord , cre recombinase , foxa2 , biology , nodal , transgene , insertional mutagenesis , genetically modified mouse , somite , genetics , microbiology and biotechnology , cre lox recombination , conditional gene knockout , phenotype , gene , embryonic stem cell , embryogenesis , mutant
Abstract During development, the organizer provides instructive signals to surrounding cells as well as contributing cells to axial structures. To dissect organizer function at different developmental stages, conditional approaches such as the Cre/loxP system for conditional mutagenesis are particularly useful. Here we describe two new Cre transgenic mouse lines, Foxa2 NFP‐Cre and Nodal PNC‐Cre, with activity in two organizer domains, the posterior notochord (PNC) and notochord. These lines were made using defined regulatory elements from the Foxa2 and Nodal genes that direct Cre expression in overlapping domains of the PNC and notochord. Our detailed analysis of the timing and location of Foxa2 NFP‐Cre and Nodal PNC‐Cre activity indicates that these lines are appropriate for conditional mutagenesis of genes expressed from early somite stages onward. genesis 45:729–736, 2007. Published 2007 Wiley‐Liss, Inc.