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Generation of conditional knockout alleles for PDGF‐C
Author(s) -
Wu Xiaoli,
Ding Hao
Publication year - 2007
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20339
Subject(s) - platelet derived growth factor receptor , conditional gene knockout , biology , allele , null allele , microbiology and biotechnology , genetics , platelet derived growth factor , exon , growth factor , embryonic stem cell , gene , phenotype , receptor
PDGF‐C is a newly identified member of the platelet‐derived growth factor (PDGF) family, which is involved in multiple cellular functions by signaling through PDGF receptor (PDGFR)‐αα and αβ dimers. PDGF‐C deficiency is perinatal lethal due to the formation of cleft palate. To further characterize the cellular function of PDGF‐C during both embryonic and postnatal development, we have generated two conditional alleles of the Pdgf‐c gene in which two lox P sites flank exon 5. Global Cre‐mediated excision of the floxed exon 5 in these alleles resulted in a complete loss of PDGF‐C expression and caused embryonic defects identical to those previously described for the PDGF‐C null embryos. These conditional alleles will therefore be the important genetic tools for dissecting the spatial and temporal roles of PDGF‐C during development and in adult tissues. Furthermore, from this work, we have also described a simple approach for creating mouse conditional alleles in an efficient manner. genesis 45:653–657, 2007. © 2007 Wiley‐Liss, Inc.