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A global double‐fluorescent Cre reporter mouse
Author(s) -
Muzumdar Mandar Deepak,
Tasic Bosiljka,
Miyamichi Kazunari,
Li Ling,
Luo Liqun
Publication year - 2007
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20335
Subject(s) - green fluorescent protein , cell sorting , biology , reporter gene , microbiology and biotechnology , cre recombinase , cell , gene knockin , transplantation , yellow fluorescent protein , transgene , flow cytometry , genetically modified mouse , gene , genetics , gene expression , medicine , surgery
The Cre/loxP system has been used extensively for conditional mutagenesis in mice. Reporters of Cre activity are important for defining the spatial and temporal extent of Cre‐mediated recombination. Here we describe mT/mG , a double‐fluorescent Cre reporter mouse that expresses membrane‐targeted tandem dimer Tomato (mT) prior to Cre‐mediated excision and membrane‐targeted green fluorescent protein (mG) after excision. We show that reporter expression is nearly ubiquitous, allowing visualization of fluorescent markers in live and fixed samples of all tissues examined. We further demonstrate that mG labeling is Cre‐dependent, complementary to mT at single cell resolution, and distinguishable by fluorescence‐activated cell sorting. Both membrane‐targeted markers outline cell morphology, highlight membrane structures, and permit visualization of fine cellular processes. In addition to serving as a global Cre reporter, the mT/mG mouse may also be used as a tool for lineage tracing, transplantation studies, and analysis of cell morphology in vivo. genesis 45:593–605, 2007. © 2007 Wiley‐Liss, Inc.