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Generation of an Frs2α conditional null allele
Author(s) -
Lin Yongshun,
Zhang Jue,
Zhang Yongyou,
Wang Fen
Publication year - 2007
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20327
Subject(s) - null allele , cre recombinase , biology , allele , exon , gene targeting , fibroblast growth factor receptor , embryonic stem cell , gene , microbiology and biotechnology , genetics , fibroblast growth factor , receptor , transgene , genetically modified mouse
The fibroblast growth factor (FGF) signaling family controls a broad spectrum of cellular processes in development and adult tissue homeostasis and function, which is expressed in almost all tissues at all stages. FGF receptor substrate 2 alpha (FRS2α) is an adaptor protein that recruits downstream substrates to the FGF receptor (FGFR) tyrosine kinase. Disruption of Frs2 α gene in mice abrogates activation of the mitogen‐activated protein kinase pathway by the FGFR and leads to embryonic lethality at day E7.5 post copulation. To circumvent the embryonic lethality resulting from disruption of the Frs2 α gene, which hinders further characterization of the role of FRS2α in adult tissue function and homeostasis, we generated an Frs2 α conditional null allele for temporally‐ and tissue‐specific disruption of the Frs2 α gene. Using gene targeting in mouse embryonic stem cells, we introduced two loxP sites flanking the largest coding exon, exon 5, in the Frs2 α allele. Our results indicate that the floxed Frs2 α ( Frs2 α flox ) allele is a true conditional null allele that encodes wildtype activity and is converted to a null allele after Cre recombinase mediated recombination. genesis 45:554–559, 2007. © 2007 Wiley‐Liss, Inc.