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A transgenic mouse Class‐III β tubulin reporter using yellow fluorescent protein
Author(s) -
Liu Li,
Geisert Eldon E.,
Frankfurter Anthony,
Spano Anthony J.,
Jiang Chloe Xue,
Yue Junming,
Dragatsis Ioannis,
Goldowitz Dan
Publication year - 2007
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20325
Subject(s) - green fluorescent protein , fluorescence , transgene , fluorescent protein , genetically modified mouse , biology , tubulin , microbiology and biotechnology , yellow fluorescent protein , class (philosophy) , microtubule , genetics , gene , physics , quantum mechanics , artificial intelligence , computer science
A yellow fluorescence protein (YFP) reporter construct was cloned downstream of the β‐tubulin III promoter and injected to produce two founder lines of transgenic mice. YFP expression was observed in many regions of the developing peripheral and central nervous system. YFP expression was first observed in the peripheral and central nervous system as early as embryonic day 9.0. There was a dramatic increase in the number of neuronal systems expressing YFP through P0. Then as the animals reached adult age, the expression levels decreased, but many neurons still show YFP expression, notably in regions of the brain undergoing adult neurogenesis, i.e., the rostral migratory stream and subgranular layer of the dentate gyrus. This reporter‐based staining was compared with anti‐class‐III β‐tubulin immunocytochemistry and shown to closely parallel the expression of the endogenous protein. These transgenic lines should provide unique models to study in vivo and in vitro neurodevelopment. genesis 45:560–569, 2007. © 2007 Wiley‐Liss, Inc.