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Cell autonomous sorting and surface positioning in the formation of primitive endoderm in embryoid bodies
Author(s) -
Rula Malgorzata E.,
Cai Kathy Q.,
Moore Robert,
Yang DongHua,
Staub Cory M.,
Capochichi Callinice D.,
Jablonski Sandra A.,
Howe Philip H.,
Smith Elizabeth R.,
Xu XiangXi
Publication year - 2007
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20298
Subject(s) - endoderm , embryoid body , microbiology and biotechnology , embryonic stem cell , biology , adult stem cell , genetics , gene
The differentiation and formation of the primitive endoderm in early embryos can be mimicked in vitro by the aggregation of embryonic stem cells to form embryoid bodies. We present morphological evidence that primitive endoderm cells often first locate in the interior of embryoid bodies and subsequently migrate to the surface. Cell mixing experiments indicate that surface positioning is an intrinsic property of endoderm epithelial cells. Moreover, Disabled‐2 (Dab2) is required for surface sorting and positioning of the endoderm cells: when Dab2 expression was eliminated, the differentiated endoderm epithelial cells distributed throughout the interior of the embryoid bodies. Surprisingly, E‐cadherin is dispensable for primitive endoderm differentiation and surface sorting in embryoid bodies. These results support the model that primitive endoderm cells first emerge in the interior of the inner cell mass and are subsequently sorted to the surface to form the primitive endoderm. genesis 45: 327–338, 2007. Published 2007 Wiley‐Liss, Inc.