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Comparative analysis of Hoxa5 allelic series
Author(s) -
Tabariès Sébastien,
Lemieux Margot,
Aubin Josée,
Jeannotte Lucie
Publication year - 2007
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20292
Subject(s) - homeotic gene , biology , hox gene , allele , null allele , genetics , mutation , mutant , phenotype , gene , gene expression
Analysis of the Hoxa5 −/− mutants has revealed the critical role of Hoxa5 in survival, specification of axial identity, and ontogeny of organs, including the respiratory tract. The presence of the selection cassette in the original Hoxa5 −/− mutation may interfere with the interpretation of the phenotypes. To circumvent this aspect and to bypass the lethality of the Hoxa5 mutation, we have designed a conditional approach and generated Hoxa5 allelic variants. The conditional allele ( Hoxa5 floxed ) behaves as a wild‐type allele. In contrast, both the Hoxa5 Δ and the Hoxa5 floxneo alleles are characterized by the loss of the functional transcript and protein, the lethality due to lung defects and the skeletal homeotic transformations similar to those of the Hoxa5 −/− mutants. Analysis of neighboring Hox gene expression patterns in the Hoxa5 mutants produced further confirmed that the Hoxa5 allelic variants are true null alleles. genesis 45:218–228, 2007. © 2007 Wiley‐Liss, Inc.