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High‐throughput enhancer trap by remobilization of transposon Minos in Ciona intestinalis
Author(s) -
Awazu Satoko,
Matsuoka Terumi,
Inaba Kazuo,
Satoh Nori,
Sasakura Yasunori
Publication year - 2007
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20290
Subject(s) - ciona intestinalis , enhancer trap , enhancer , ciona , biology , transposable element , insertional mutagenesis , transposase , sleeping beauty transposon system , genetics , minos , genome , gene , reporter gene , transgene , mcherry , transposon mutagenesis , gene expression , green fluorescent protein , physics , nuclear physics , neutrino , neutrino oscillation
The enhancer trap approach utilizing transposons yields us information about gene functions and gene expression patterns. In the ascidian Ciona intestinalis , transposon‐based transgenesis and insertional mutagenesis were achieved with a Tc1/ mariner transposon Minos . We report development of a novel technique for enhancer trap in C. intestinalis . This technique uses remobilization of Minos in the Ciona genome. A Minos vector for enhancer trap was constructed and a tandem array insertion of the vector was introduced into the Ciona genome to create a mutator line. Minos was remobilized in Ciona chromosomes to create new insertions by providing transposases. These transposase‐introduced animals were crossed with wild‐type animals. Nearly 80% of F1 families showed novel GFP expression patterns. This high‐throughput enhancer trap screen will be useful to create new marker transgenic lines showing reporter gene expression in specific tissues and to identify novel patterns of gene expression. genesis 45:307–317, 2007. © 2007 Wiley‐Liss, Inc.