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Double‐inducible gene activation system for caspase 3 and 9 in epidermis
Author(s) -
Shah Viraj R.,
Koster Maranke I.,
Roop Dennis R.,
Spencer David M.,
Wei Lei,
Li Qi,
Schwartz Robert J.,
Chang Jiang
Publication year - 2007
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20288
Subject(s) - transgene , gene , gene expression , biology , microbiology and biotechnology , inducer , computational biology , chemistry , genetics
Abstract Expression of genes with tight and precise temporal and spatial control is desired in a wide variety of applications ranging from cultured cells and transgenic animals to gene therapy. While current inducible systems, such as RU486 and chemical inducers of dimerization (CID), have improved earlier inducible models (Gossen et al ., 1995, Science. 268:1766–1769; Wang et al ., 1994, Proc Natl Acad Sci USA 91:8180–8184), no single system is perfect at present. One potential drawback of these systems is leakage of transgene expression, causing limitations of each system. We have developed an inducible model containing both RU486 and CID systems, which in addition to inducing caspase activation, has potential applicability specifically to other genes encoding proteins that require a dimerization event for activation. This Double‐Inducible Gene Activation System generates two barriers for the target gene expression and protein activation thereby minimizing leakage. genesis 45:194–199, 2007. Published 2007 Wiley‐Liss, Inc.