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Generation of a floxed allele of the mouse endoglin gene
Author(s) -
Allinson Kathleen R.,
Carvalho Rita L.C.,
van den Brink Stieneke,
Mummery Christine L.,
Arthur Helen M.
Publication year - 2007
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20284
Subject(s) - endoglin , biology , allele , phenotype , angiogenesis , telangiectases , cancer research , genetics , gene , pathology , medicine , telangiectasia , stem cell , cd34
Endoglin is an auxiliary receptor for TGFβ signalling. Heterozygous germline Endoglin mutations have been identified in patients with the vascular abnormality, Hereditary Haemorrhagic Telangiectasia. Endoglin is upregulated in endothelial cells during angiogenesis and loss of Endoglin in the mouse results in embryonic lethality at mid‐gestation. This phenotype points to an important role of Endoglin in new blood vessel formation but precludes analysis at later stages in development and in postnatal life. To bypass this limitation and allow further investigations of the function of Endoglin we have generated a floxed Endoglin allele in which loxP sites flank exons 5 and 6. Mice homozygous for this allele are normal and in the presence of appropriate Cre lines will allow time and cell specific Endoglin deletion for in vivo analysis of function in cardiovascular development and disease. genesis 45:391–395, 2007. © 2007 Wiley‐Liss, Inc.