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High‐resolution mapping of the Gli3 deletion in the mouse extra‐toes H mutant
Author(s) -
Genestine Matthieu,
Robert Benoît,
Lallemand Yvan
Publication year - 2007
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20270
Subject(s) - gli3 , mutant , allele , mutation , biology , genetics , null allele , gene , deletion mapping , microbiology and biotechnology , high resolution , chromosome , gene expression , repressor , remote sensing , geology
Extra‐toes is a semidominant mutation that affects the Gli3 gene and provokes limb and brain abnormalities. Among the different alleles of this mutation, Xt H is due to a deletion that has not yet been fully characterized. Using a PCR‐based strategy, we undertook a high‐resolution mapping of this deletion and confirmed that Xt H is a null allele of Gli3 . We further designed a PCR test to identify unequivocally heterozygous and homozygous embryos from their wild‐type littermates. Despite the length of the Xt H deletion, available data on the mouse genome indicate that no genes other than Gli3 are deleted in Xt H mutants. Thus, the Xt H mutation can be used as a model for studying the effects that absence of Gli3 function has during development. genesis 45:107–112, 2007. © 2007 Wiley‐Liss, Inc.