Premium
Ubiquitous and uniform in vivo fluorescence in ROSA26‐EGFP BAC transgenic mice
Author(s) -
GielMoloney Maryann,
Krause Daniela S.,
Chen Gang,
Van Etten Richard A.,
Leiter Andrew B.
Publication year - 2007
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20269
Subject(s) - green fluorescent protein , transgene , biology , genetically modified mouse , microbiology and biotechnology , in vivo , bacterial artificial chromosome , transplantation , chimera (genetics) , fate mapping , reporter gene , flow cytometry , gene , gene expression , stem cell , genetics , progenitor cell , medicine , surgery , genome
Transplantation studies and cell lineage analyses require the ability to explicitly distinguish morphologically identical cells that have an identifiable marker indicating their origin in vivo. Several reporter mouse strains have been generated for such studies, but pancellular detection of the marker in all tissues has not been achieved. In this report, we describe the generation of transgenic mice that express enhanced green fluorescent protein (EGFP) under control of a 187 kb bacterial artificial chromosome (BAC) containing the murine ROSA26 locus, and show several advantages over existing EGFP reporter lines. It is demonstrated that EGFP is ubiquitously and reproducibly expressed from the murine BAC transgene in all organs and tissues analyzed, including the hematolymphoid compartment. Using this new reporter strain in hematopoietic cell transplantation studies, it is demonstrated that leukocytes in recipients maintain uniform transgene expression and are easily distinguished by flow cytometric analysis of live cells. The results suggest that the ROSA26 BAC is an efficient strategy for expressing complex transgene cassettes in vivo. genesis 45:83–89, 2007. Published 2007 Wiley‐Liss, Inc.