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Targeting iCre expression to murine progesterone receptor cell‐lineages using bacterial artificial chromosome transgenesis
Author(s) -
Mukherjee Atish,
Soyal Selma M.,
Wheeler David A.,
FernandezValdivia Rodrigo,
Nguyen Jonathan,
DeMayo Francesco J.,
Lydon John P.
Publication year - 2006
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20257
Subject(s) - transgenesis , recombineering , bacterial artificial chromosome , transgene , biology , cre recombinase , embryonic stem cell , genetics , genetically modified mouse , computational biology , microbiology and biotechnology , progesterone receptor , gene , homologous recombination , reproductive technology , genome , embryogenesis , cancer , estrogen receptor , breast cancer
Gene‐targeting in embryonic stem cells has been the dominant genetic approach when engineering mouse models to query the physiologic importance of the progesterone receptor (PR). Although these models have been instrumental in disclosing the in vivo significance of the progesterone signaling pathway, generation of such mice exacts considerable expenditure of time, effort, and expense. Considering the growing list of new PR mouse models that are urgently required to address the next questions in progestin biology, bacterial artificial chromosome (BAC) recombineering in conjunction with transgenesis was evaluated as an alternative method to accelerate the creation of these models in the future. Using this approach, we describe the generation of three PR‐BAC iCre transgenic lines in which improved Cre recombinase (iCre) was targeted in‐frame, downstream, and under the control of the PR promoter contained within a BAC transgene. Crossing with the ROSA26R revealed that the PR‐BAC iCre transgenic expresses active iCre only in cell‐lineages that express the PR. The specificity of the PR‐BAC iCre transgene not only underscores the importance of BAC‐mediated transgenesis as a quick, easy, and affordable method by which to engineer the next generation of PR mouse models, but also provides a unique opportunity to investigate transcriptional control of PR expression as well as PR structure‐function relationships in vivo. genesis 44:601–610, (2006). Published 2006 Wiley‐Liss, Inc.