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Expression of an Nkx3.1‐CRE gene using ROSA26 reporter mice
Author(s) -
Stanfel M.N.,
Moses K.A.,
Carson J.A.,
Zimmer D.B.,
DeMayo F.,
Schwartz R.J.,
Zimmer W.E.
Publication year - 2006
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20250
Subject(s) - biology , microbiology and biotechnology , gene , reporter gene , gene expression , genetics
The genetic locus of Nkx3.1 , an early murine marker of sclerotome and prostate development, was disrupted by a knock in of CRE recombinase via homologous recombination in embryonic stem cells. Cell fate mapping revealed previously unidentified cell lineages expanded from Nkx3.1 ‐expressing cell populations and recapitulated reported Nkx3.1 expression patterns. In lineage trace experiments of E18.5 Nkx3.1‐CRE ; R26R embryos novel staining was observed in areas of the lungs, portions of the duodenum, and vertebral elements of the skeleton. β‐galactosidase activity measured in Nkx3.1‐CRE ; R26R and Nkx3.2‐CRE ; R26R embryos was observed in overlapping regions of the sclerotome but no apparent change in Nkx3.1 expression was seen in the Nkx3.2 mutants by in situ hybridization. genesis 44:550–555, 2006 Published 2006 Wiley‐Liss, Inc.