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Sfrp5 is not essential for axis formation in the mouse
Author(s) -
Leaf Irina,
Tennessen Jason,
Mukhopadhyay Mahua,
Westphal Heiner,
Shawlot William
Publication year - 2006
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20248
Subject(s) - endoderm , foregut , biology , wnt signaling pathway , morphogenesis , dkk1 , microbiology and biotechnology , primitive streak , phenotype , gastrulation , genetics , embryogenesis , embryo , gene , anatomy , signal transduction , embryonic stem cell
Secreted frizzled related protein ( Sfrp ) genes encode extracellular factors that can modulate Wnt signaling. During early post‐implantation mouse development Sfrp5 is expressed in the anterior visceral endoderm (AVE) and the ventral foregut endoderm. The AVE is important in anterior–posterior axis formation and the ventral foregut endoderm contributes to multiple gut tissues. Here to determine the essential role of Sfrp5 in early mouse development we generated Sfrp5 ‐deficient mice by gene targeting. We report that Sfrp5 ‐deficient mice are viable and fertile. To determine whether the absence of an axis phenotype might be due to genetic redundancy with Dkk1 in the AVE we generated Sfrp5 ; Dkk1 double mutant mice. AVE development and primitive streak formation appeared normal in Sfrp5 −/− ; Dkk1 −/− embryos. These results indicate that Sfrp5 is not essential for axis formation or foregut morphogenesis in the mouse and also imply that Sfrp5 and Dkk1 together are not essential for AVE development. genesis 44:573–578, 2006. Published 2006 Wiley‐Liss, Inc.