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Broad‐Complex , E74 , and E75 early genes control DNA puff BhC4‐1 expression in prepupal salivary glands
Author(s) -
Basso L.R.,
Neves M. de C.,
Monesi N.,
PaçóLarson M.L.
Publication year - 2006
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20239
Subject(s) - ecdysone , drosophila melanogaster , biology , gene , salivary gland , gene isoform , transcriptional regulation , ecdysone receptor , microbiology and biotechnology , genetics , gene expression , transcription factor , biochemistry , nuclear receptor
The DNA puff BhC4‐1 gene of the sciarid Bradysia hygida is induced in salivary glands prior to the pupal molt as a secondary response to the increase in ecdysone titers. Previous studies demonstrated that the BhC4‐1 promoter is activated in transgenic Drosophila melanogaster salivary glands as a late response to the ecdysone peak that triggers metamorphosis, revealing that this aspect of BhC4‐1 transcriptional regulation is conserved in the Drosophila background. To identify regulators of BhC4‐1 expression, we utilized a candidate gene approach and tested the roles of the ecdysone‐induced genes BR‐C , E74 , and E75 . Our results reveal that the BR‐C Z3 isoform is essential for BhC4‐1‐lacZ induction in prepupal salivary glands and constitute the first demonstration of the participation of early genes products on DNA puff genes regulation. genesis 44:505–514, 2006. © 2006 Wiley‐Liss, Inc.